This machine is on 24/7 but I think it will run as soon as it comes back up. I have seen backup running while I was doing other stuff. This is just an imaging program. If you have an infected file, I think it will faithfully be copied ... infected. That is important to remember if you think you have a virus. The virus will still be in that image so when you are restoring, be sure you go back far enough to predate the infection.
